Alekhya Josyula Venkata

5 Questions 7 Answers 0 Followers

Questions related from Alekhya Josyula Venkata

Has anyone immunized mice with Influenza HA1 antigen?

I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction...

08 June 2016 3,859 3 View

What is the best way to identify protein in FPLC fractions?

I subject NiNTA purified proteins to size exclusion chromatography to get rid of any impurities and identify monomers. I'm not entirely sure what the best approach is for further downstream...

02 March 2016 8,843 5 View

What are the advantages of detecting memory B cells using ELISPOT instead of flow cytometry?

I plan to do an immunization and look at memory B cells among other B cell subsets. I will be doing a flow cytometric analysis (I will use cell surface markers and also look at antigen binding...

27 September 2015 7,512 3 View

Does anyone have experience looking at t follicular helper cells and b cells using flow cytometry?

I stained mouse lymph nodes with pd-1,cxcr5,cd4 and cd44 for t follicular helper cells and cd19,cd38,cd73 and af488 conjugated to an antigen that I originally immunized the mice with. I collected...

23 March 2015 6,544 1 View

Why is capture ELISA assay not detecting not working or capture HIS-tagged GP120?

I tried to detect HIS tagged gp120 using capture ELISA. There was no color development. Could it be that the protein I purified was not gp120? The gp120 was NiNTA purified and has a PADRE...

22 March 2015 7,170 4 View