7 Questions 9 Answers 0 Followers
Questions related from Alekhya Josyula Venkata
I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction...
08 June 2016 4,518 3 View
I subject NiNTA purified proteins to size exclusion chromatography to get rid of any impurities and identify monomers. I'm not entirely sure what the best approach is for further downstream...
02 March 2016 9,031 5 View
I plan to do an immunization and look at memory B cells among other B cell subsets. I will be doing a flow cytometric analysis (I will use cell surface markers and also look at antigen binding...
27 September 2015 7,710 3 View
I am trying to plan my immunization protocol based on what I see in the literature. I am particularly interested in the time points previous studies have chosen to monitor their study animals.
05 May 2015 3,051 4 View
It was my understanding that it represents the percentage of cells within the gate, when I changed the decimal settings to include three numbers after the decimal pints, the values alter. Can...
30 March 2015 7,267 3 View
I stained mouse lymph nodes with pd-1,cxcr5,cd4 and cd44 for t follicular helper cells and cd19,cd38,cd73 and af488 conjugated to an antigen that I originally immunized the mice with. I collected...
23 March 2015 6,788 1 View
I tried to detect HIS tagged gp120 using capture ELISA. There was no color development. Could it be that the protein I purified was not gp120? The gp120 was NiNTA purified and has a PADRE...
22 March 2015 7,333 4 View
