Pedro A Reche

9 Questions 65 Answers 0 Followers

Questions related from Pedro A Reche

Tetatus and diphteria toxoids in vaccines?

I read a while ago that tetatus and diphtheria toxoids only represent about 50% of proteins in vaccines but I cannot find the original reference. Please, if someone knows about it share the info.

07 February 2023 1,504 4 View

How do I restrict a blast search on the command line to a taxonomic group?

I am using blast+ tools on the command line with local databases. I would like to do a blast against nr limiting the search to a given taxon, just as one can do in the blast web. Is that...

09 April 2015 2,505 5 View

How to correct negative branches from neighbor-joining method?

I am getting negative branches with the Neighbor-joining method, which I set to zero. However, I've read that I should transfer negative distances somewhere else and I do not know how. Does...

23 February 2015 3,853 4 View

Aligning a protein sequence onto a structure-based multiple sequence alignment ?

I am trying to align a sequence to a multiple sequence alignment obtained from an 3D-structural alignment (superimposed 3D structures). The sequence is related to the sequences in the multiple...

30 May 2013 8,452 15 View

Companies for ordering Custom Synthetic Peptides

We need synthetic peptides for Elispot assays and we wonder from which company to get them. We have found that ChinaPeptides offer peptide synthesis at very competitive prices. However, we have...

03 April 2013 6,463 3 View

Why are clustal and Phylip Bootstrap N-J trees different?

ClustalX has an option to obtain bootstrap neighbor joining trees from an alignment. However, the trees differ considerably from those one can get using bootstrapping with the phylip package...

17 January 2013 2,592 13 View

Th1/Th2 polarization in peripheral tissues?

I understand Th1/Th2 T cell polarization occurs in secondary lymphoid organs (e.g. lymphatic nodules), where T cells recognized the antigens in the presence of different signals that condition...

07 September 2012 8,239 17 View

Inhibition of B cell response by FcgRIIB-I

FcgRII-I is an inhibitory receptor expressed in both naive and memory B cells. However, in naive B cells, this receptor prevent the response against antigens for which there are preformed...

27 April 2012 4,573 3 View

Do RSS pair during V(D)J segment rearrangement ?

We are having here a discussion over a scientific topic and I thought I can get here the help to settle it. The diversity of the immunoglobulins is generated by stochastic rearrangement of...

25 April 2012 8,314 12 View