Is a wall with constant pressure and constant inlet flow similar to an inlet boundary?

At an opening in turbulent flow, using wall boundary condition, with constant flow rate and constant pressure inlet, would be similar to an opening with inflow BC?

03 February 2021 2,390 5 View

Is it possible to estimate the most recent common ancester using o & 1 data? If yes would you please introduce a software?

Dear professors Respectfully, i know that sequences can help us to reveal the most common ancestor in phylogenetic studies. However, in genetic diversity studies using IRAP, SSR, markers etc, i...

13 November 2019 9,305 6 View

Identify DNA bands transfer from gel to the membrane(southern blotting)?

Hello Is there a way to detect transferring of DNA bands from gel to membrane in southern blotting without staining? Thanks

10 November 2017 7,804 3 View

Can anyone suggest the best method for measuring PON1 arylesterase activity in serum?

05 January 2015 3,060 1 View

Is there a possibility to see any green substance in extracted DNA?

I saw a green substances in potamogeton DNA. Does anybody know anything about this?

10 May 2014 5,702 4 View

What is the cause of green DNA in Potamogeton extracted DNA and how can I delete that?

Green DNA of Potamogeton and deleting the color.

05 May 2014 9,649 3 View

Hydrological test statistics

What is the best test statistic for gamma distribution?

01 January 1970 7,770 3 View

What is the best RMSE value for rainfall data?

rmse and mad

01 January 1970 8,622 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View