07 January 2015 2 6K Report

I am going to measure this activity but I don't know the exact protocol (quantity and concentration of PA or CaCl2...)

More Atena Raee's questions See All
Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

Can anyone offer advice on how to show the inhibition zone of bacillus against s.aureus?

Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...

26 May 2024 2,151 6 View

Why the sequence of my PCR poduct is very clear without any noise, but very short in size?

I faced with a problem, and I think you can help me regarding this issue. I have sent one of my PCR poducts (ITS1-ITS4) to a company in order to sequence it. Indeed, I sent them various PCR...

01 February 2024 2,626 3 View

Why did I observe more than one band in my PCR product?

What is your solution regarding extra bands in ITS molecular markers? I am trying to use ITS1 and ITS4 markers in 15 plant species. Unfortunately, I only received extra bands instead of one after...

28 November 2023 9,305 4 View

What is PCR bias?

I've recently faced a question that I couldn't solve on my own or with colleagues. I want to know what are PCR biases and how to handle them? Any answer in welcome.

20 June 2023 9,275 1 View

Questions about the bacterial taxonomy ?

Hi all, I have two questions regarding taxonomy 1- how do you define a novel family? or even order? 2- if you have a strain that is not deposited in two culture collection, can you describe it...

20 June 2023 6,362 1 View

Dear professors, would you please let me know in which case it is possible to synonymized taxa?

I have two morphologically similar species that differs molecularly only by 4 bases. Can I synonymmized them? If not, how many base differences can lead to this taxonomic revision? How do I deal...

10 April 2023 3,602 18 View

Gram negative inhibitor in culture media?

I want to isolate only yeasts from a sample and I want to know if there is (a) compound/compounds that I can use to inhibit the growth of gram-negative bacteria in my culture media. If that...

06 October 2021 2,978 6 View

Precipitation Issue with Rotenone?

Has anybody had any issues with Rotenone precipitating out in cell culture medium. I see brown precipitates forming in cell culture media after 1-2days in culture at doses above 30uM. If so, what...

28 July 2021 4,002 0 View

DNA purification post extraction for kombucha?

I extracted DNA from some kombucha products with a manual protocol, with one final step using phenol, for 16S and ITS sequencing. However, there is a problem for the majority of my samples in PCR...

12 June 2021 9,655 3 View

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Is someone interested in testing some of our new Alluaudite compounds (Molybdate) in the fields of heterogeneous catalysis and photocatalysis?

We have an original Alluaudite-Molybdate we want to test for catalysis and photocatalysis applications.

04 August 2024 2,261 1 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

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Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

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