Can anyone suggest me an efficient Yeast-2-Hybrid Kit for testing protein-protein interaction?

22 November 2021 1 2K Report

Hello! I am looking for a yeast-2-hybrid kit to buy for testing protein-protein interaction. By kit I mean something that comes with the vectors, the controls as well as the yeast strains. I have been using the LexA and ACT2.2 vectors and L40 yeast strain but since I'm facing some issues with the system, I was planning to buy a similar kit from a trusted source/company. I want to mention that I'm not doing library screens but just want to test protein-protein interactions between different proteins.

Adwaita Prasad Parida

takara or clontech is good (the matchmaker system) if want to screen library,

if u want to screen predicted proteins (one to one) then u dont need.

just buy vector strain and media

Badges
Science topic

Similar topics
Clinical Pharmacology
Dosing

More Shreyosi Bose's questions See All

Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

Is it okay to use PCA regression on a time series dataset?

I have a dataset of a binary response variable and 22 predictor variables that have multicollinearity. My goal is to make a predictive model. The data was sampled every 30 seconds over three days....

08 October 2019 5,746 2 View

How to do time series analysis with one/few missing data points?

I have three datasets given to me of images of a conveyor belt taken every 30 seconds over 3 days, but one image was removed/lost in one of them and a few (~10-20) in the others. I calculated the...

02 October 2019 3,893 5 View

Do you think fruit sugar is harmful for human health?

We hear regularly from health organization and experts that we should eat less sugar. As we know that fruit is also valuable source of sugar. So, consumption of more fruit is harmful for human...

22 September 2019 6,270 6 View

How to culture cancer cells the right way?

I am relatively new to cell culturing and I am currently working with several lines of cancer cells, some of them being HeLa, A549, HCT116, etc. I have some very basic doubts regarding the...

03 September 2019 3,003 3 View

How to characterize surface roughness using stereomicroscope pictures when the two samples are different colors (one is much darker)?

I am using the stereomicroscope to generate stacks to capture each plane of the leaf in focus. I am using ImageJ and the plugins SurfCharJ 1q and Fraclac to quantify the leaf roughness and fractal...

04 July 2019 2,282 3 View

Why are XRD images black and white?

XRD images or diffraction spots are black and white. SEM images too. In fact, I think any atomic scale image is black and white. Why are they colorless?

10 April 2019 4,563 3 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 7,547 3 View

How to estimate the marginal effects of Bivariate Probit Models in STATA?

Dear All, I am running a Bivariate Probit model in STATA and tried to estimate the marginal effects; however, every time its showing some error message. Could you please help me to run the exact...

25 March 2019 4,637 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View