Hi, you can simply use R:

https://www.r-project.org/

Tutorial:

http://www.r-bloggers.com/r-tutorial-series-two-way-anova-with-pairwise-comparisons/

Dear Colleague,

The statistical analyses can be  performed using the two following softwares: PRISM v 6.01 for Windows (GraphPad Software Inc., www.graphpad.com) and MINITAB (Version 16, Penn State College, PA, USA).

Best regards N. Soltani

Try PAST, open access 

Also Excel will do the job!