Brigitte Bruijns

2 Questions 14 Answers 0 Followers

Questions related from Brigitte Bruijns

My dsDNA sample might be degraded and might contain ssDNA as well. How can I easily check (and quantify) if my DNA is stil double stranded?

I have checked a sample of salmon DNA (provided as 'solid' dsDNA and brought into TE-buffer up to a certain concentration) with the Nanodrop. According to the measurment (performed several times),...

05 April 2016 9,313 4 View

Additional staining of the gel after real-time PCR?

When I perform a real-time PCR experiment (with for instance SYBR Green or EvaGreen), would you also stain the gel (to check the amplicon lenght) with this dye or is the presence of the dye from...

23 March 2016 1,307 8 View