Influence of COVID on the mental health - Survey?

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14 April 2020 191 3 View

Can anyone say about to make a perfect way to make GO bases Nanocomposite?

I am working in ternary composites. Is there anyone working in graphene and its derivatives. I want to make a composite comprised of GO with some inorganic material.

05 May 2019 6,642 3 View

Can anyone say clearly about the calculation for doping metal oxides using some different mol ratios?

I need to dope metal to my metal oxide matrix, Can anyone clearly explain me about the calculations that i need to workout for mol %.

26 March 2019 1,277 4 View

Is there any medium to support the growth of Streptomycetes only?

Does the growth of microbes on ISP4 medium confirm the organisms to be a streptomycete? Kindly suggest some selection medium for Streptomycetes

17 February 2019 7,129 3 View

Air pollutant accumulated Filter paper preservation?

Usually the filter papers will be desiccated for 24 hr before and after ambient air pollutant sampling. As we encounter some error in weighing balance, we are unable to weigh the exposed air...

22 October 2018 1,718 4 View

What is the reason of decrease the bandgap when decrease the cystallite size?

I prepared nanorod structured undoped (pure) oxide thin film by spray pyrolysis method function of different substrate temperatures (STs). I found that decrease the bandgap when decreases the...

08 August 2017 5,868 21 View

Which online software is reliable for Protein Transmembrane helix prediction

25 May 2017 6,484 4 View

What are the existing "Quality Assessment Models" available for building or general construction?

Similar to CONQUAS (Singapore) and PASS (HK), Is there any other quality assessement models available in the practice or in the literature domain?. Thanks Marimuthu K

26 October 2016 6,584 4 View

Double band for a single protein on western blot?

Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...

16 December 2015 5,351 12 View

How to select a correct primer for an unknown organism for PCR?

We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...

29 March 2014 1,563 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View