Anandharaj Marimuthu

8 Questions 13 Answers 0 Followers

Questions related from Anandharaj Marimuthu

How to calculate the ligand binding energy using UCSF chimera?

I have used the SwissDock to perform the docking with three different ligands, i would like to calculate the binding energy of each ligand. But I don't know how to calculate it using UCSF Chimera....

14 January 2017 3,988 3 View

Can anyone suggest me a best transformation protocol for Bacillus subtilis using electroporation?

I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...

21 December 2015 6,195 1 View

Why do living organisms use mainly L-form of amino acids and D-forms of sugars?

I want to know why the organisms used L form amino acids instead of D form amino acids and why they cannot use L form sugars..

15 September 2014 3,653 1 View

Why do we choose 16S rRNA instead of others to identify microorganisms?

I want to know the reason why we particularly use 16S rRNA for identifying species level.

21 February 2014 5,954 22 View

How to calculate enzyme activity in U/ml?

After the enzyme assay we need to calculate the enzyme activity in the form of U/ml. is there any formulae is available for this

07 February 2014 9,908 4 View

Protocol for Keratinase assay?

Does anybody have a protocol for keratinase assay, using keratin or azokeratin as a substrate?

19 January 2014 1,193 1 View

What is the most preferable chemical for pretreatment of biomass?

I want to know which chemical compounds are most commonly used for substrate pretreatment for biofuel production?

10 January 2014 7,026 4 View

How do I separate bacteriocin from culture broth?

In order to determine the antimicrobial activity of several lactic acid bacteria (Lactobacillus spp.), I want to separate bacteriocins from the culture broth. Can anybody suggest the most...

03 January 2014 145 7 View