Anandharaj Marimuthu

13 Questions 22 Answers 0 Followers

Questions related from Anandharaj Marimuthu

How to calculate the ligand binding energy using UCSF chimera?

I have used the SwissDock to perform the docking with three different ligands, i would like to calculate the binding energy of each ligand. But I don't know how to calculate it using UCSF Chimera....

14 January 2017 3,996 3 View

Can anyone suggest me a best transformation protocol for Bacillus subtilis using electroporation?

I would like to transform my episomal plasmid to Bacillus subtilis, i have many transformation protocol but im confused about which one to choose, if anyone already succeed to use any optimized...

21 December 2015 6,204 1 View

Can anyone suggest me a loading control (for western blot) for yeast secretary proteins?

i want to detect my his tagged protein in the yeast culture supernatant using western blot, i want to use loading control but i'm little confused about which loading control i should use. i...

17 December 2015 231 3 View

How can I convert the Agarose Gel image for publication?

I would like to know how to convert the gel image from black background to white background for publication.

26 June 2015 5,297 5 View

Why do living organisms use mainly L-form of amino acids and D-forms of sugars?

I want to know why the organisms used L form amino acids instead of D form amino acids and why they cannot use L form sugars..

15 September 2014 3,660 1 View

Why do we choose 16S rRNA instead of others to identify microorganisms?

I want to know the reason why we particularly use 16S rRNA for identifying species level.

21 February 2014 5,966 22 View

How to calculate enzyme activity in U/ml?

After the enzyme assay we need to calculate the enzyme activity in the form of U/ml. is there any formulae is available for this

07 February 2014 9,916 4 View

Protocol for Keratinase assay?

Does anybody have a protocol for keratinase assay, using keratin or azokeratin as a substrate?

19 January 2014 1,199 1 View

What is the most preferable chemical for pretreatment of biomass?

I want to know which chemical compounds are most commonly used for substrate pretreatment for biofuel production?

10 January 2014 7,034 4 View

How do I separate bacteriocin from culture broth?

In order to determine the antimicrobial activity of several lactic acid bacteria (Lactobacillus spp.), I want to separate bacteriocins from the culture broth. Can anybody suggest the most...

03 January 2014 154 7 View

What is the cheapest source for biodiesel production?

I want to know which substrate is very economical and the cheapest source for biodiesel production.

31 December 2013 8,902 5 View

What is the optimum signal to inject the sample in Ion exchange chromatography (IC)?

The total signal is always varying from 10-3, so I confused which signal is the correct one for the injection. Anybody can help me?

31 December 2013 8,624 1 View

Does anybody know the Dugong dugon status in india?

Dugong dugon (sea cow) is a marine mammal, it lives in very shallow areas in the sea due to their food habitat. in india it lives in gulf of mannar, but today it faces lots of threats from human...

06 December 2013 6,721 6 View