Does anyone know of any chemicals that selectively damage fish sperm DNA without influencing other systems? like: dimethyl sulfate, ethylnitrosourea, trypaflavine?
I do not think it is possible to selectively damage sperm DAN without affecting motility and other processes as the sperm membrane is different as compared to somatic cells and is not easily permeable to may stuff and it often gets damaged in the process.
I have tried mechanical ways of inactivating the DNA..especially UV irradiation and enucleation..but DNA damage by chemicals would be an interesting area to look at
by other systems do you mean cellular functions or other organ systems (cells lines other than gametes)?
BrdU comes to mind but I am unsure if this has an effect on transcription once incorporated into the nascent genome. Seen paper showing previously down regulated genes become up regulated when BrdU is incorporated which suggests more a damage to the epigenetic expression than loss of functionality of the genes..
I cannot totally agree ..but its an interesting area to be explored..thanks
As I understand BrdU incorporates into cell DNA at the S phase of the cell cycle, so it is not quite suitable for sperm treatment. But any way thank you for the help.
Suggest selection of viable sperm following UV irradiation is easier. Waiting several hours (6-24) after irradiating the sperm allows damaged cells to perish, leaving viable cells for the experiment. I also used sperm from another species (Cyprinus carpio) to activate Micropterus salmoides eggs.
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