Hi All,
I want to select a cell line for our drug study but it has to be highly sensitive to p53 induction - i.e. overexpression of p53 can easily kill the cell line.
Any suggestion will be deeply appreciated. If you have personally worked on those cells will be even better.
Thanks,
Lyra
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In general, just overexpression of p53 in epithelial cell lines result in cell growth arrest and not cell death. Try lymphoid or myeloid cell lines.
Hello
As a rule of thumb, it is nearly impossible to keep alive a cell line with overexpression of of transgene of wild type TP53 (either human or from another species)
You will induce either a growth arrest or apoptosis depending on the cell type. Usually, but not always, lymphoid cell line will die from apoptosis. For epithelial cells , it will vary. The only way to express wt TP53 is the use of inducible promoter.
If someone give you a cell line expressing exogenous non inducible wt TP53, please ensure that a mutation did not inactivate the TP53 transgene as it is very frequent that mutated clones are selected .
If you want a cell line that express naturally wild type TP53 which is functional, it is another story as there are plenty of them. It will dependant of the reading that you want to have with your drug. Apoptosis, growth arrest, senescence ? Do you want an epithelial cell line derived from tumour such as A549 or mcf7 ? a non tumoral cell line such as MCF10A derived from a normal breast ? Lymhoid cell lines such as HG83 ?
All these cell line will have a specific phenotype upon TP53 induction. You have a lot of choice that you must tailor according to your need
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