How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

How can we differentiate crystalline peak to an amorphous peak of XRD ?

When characterized with XRD , my prepared sample show peaks which are broader(bottom) than crystalline peaks but unlike amorphous materials, have sharp top. Is there any limit of intensity or full...

15 December 2020 671 4 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

Regarding FAR, FRR, EER curves?

I have the image database, but I need to calculate the FAR, FRR, and EER and plot them. I am unable to understand how to plot them as they are the images... I am unable to make a program using the...

05 October 2020 3,459 3 View

Can anyone help me out to find SMA connector for a 5g millimeter-wave antenna of dimension 10*10 mm ?

Hello everyone. I am looking for an SMA connector for a 5g millimeter-wave antenna of dimension 10*10mm. The frequency range of operation is up to 40 GHz. If anybody from India is working in this...

29 August 2020 5,275 5 View

How to convert sdf file to pdb or mol2 file ?

I am using Autodock4 for protein-ligand interaction. I need a pdb or mol2 file for ligand. I have downloaded sdf file and convert it in pdb and mol2 file by using save as option. But these files...

23 February 2020 8,819 7 View

Wildtype plant DNA concentration and dilution?

I have 10 rice samples which I'm testing with housekeeping primers. 9 of them are supposed to be transgenic. And 1 is wildtype. I did a PCR with all of them, in which I had a band with all the...

09 February 2020 9,323 3 View

How to quantify biofilm biomass, maximum thickness, and surface to volume ratio from CLSI image, using Image J??

I tried using COMSTAT plugin in Image J, but it is unable to analyse .czi format. Can anyone help?? Or is there any other way to analyse biomass, maximum thickness, and surface to volume ratio...

03 December 2019 3,634 4 View

How do devices live in their own reality based on the information that has been derived?

This question is for my thesis which exploring the internet infrastructure, internet of things and aspects of object-oriented ontology in building device-device relationships.

22 September 2019 2,367 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View