Can anyone suggest any mesenchymal stem cell line derived from umbilical cord blood from ATCC?

More Swati Chitrangi's questions See All

What is difference between ZIF-8 and ZIF-L?

In one of my sample, there are two peaks ranged from 10º ~ 12º, suggesting the new phase of ZIF-L. What is that phase - orthorhombic or cubic? Can anyone please provide JCPDS (ICDD) card number...

22 May 2024 179 1 View

How can I determine value of n, C, and A from Randles-Sevcik equation using CV results?

I'm working with Zeolites as electrode materials that exhibit battery-like behavior in three-electrode configuration. So, I did plots of peak current versus v1/2 used to calculate the slopes for...

22 May 2024 9,193 1 View

Where I can purchase a lower molecular weight cutoff membrane?

Please provide information on where I can purchase a 150 kDa, 500 kDa, 1000 KDa and 5000 KDa molecular weight cutoff membrane? for separation of ferulic acid (194 mwt) form mixtue of sample. where...

20 March 2024 2,818 3 View

have observed an abrupt decrease in coercivity and large increase in exchange bias on decreasing temperature below 100K? why?

I have observed an abrupt decrease in coercivity and large increase in exchange bias on decreasing temperature below 100K, whereas maximum magnetisation still increases. Above 100K the coercivity...

10 January 2024 443 2 View

What precautions one should take to get accurate fractions of two phases in rietveld refinement?

I am able to fit 2 different phases for a particlular xrd pattern with chi2

19 December 2023 9,059 2 View

Is it possible to add peer review work done for journal article / review of book chapter to research Gate ?

Peer review work done for reviewing a journal article or a book doesn't get any recognition. At present only web of science permits to add reviewer's work. Is it possible to add this review work...

22 November 2023 6,675 1 View

Why there is only thioredoxin protein is expressed and not my protein in pET32b vector?

I am trying to purify a protein using thioredoxin tag. The clone I made is in pET32b vector having N-terminal thioredxin tag and C-terminal His-tag. I already checked the plasmid using...

06 November 2023 9,339 3 View

Sometimes the specific surface area of synthesized MOF electrode material is large but its capacitance value calculated from GCD is small, Why?

It contradict the statement that capacitance increase with increment in specific surface area and decrement in pore size.

25 September 2023 9,733 1 View

I am doing a PCR for a 650 bp DNA for cloning purpose but getting a PCR product of 400 bp even with different conditions. what can be the reason?

1.Non-specific band at 400 bp, even though by gene size is 650 bp 2.No primer dimer 3.PCR Conditions using right now: Denaturation - 98 degrees (45 sec), ext.- 98 degrees (30 sec)...

24 September 2023 6,972 2 View

How to determine the load rate and load for nanoindentation?

I am not able to find out the condition through the help of literature.

28 August 2023 7,709 3 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View