Can anyone suggest an efficient method for miRNA overexpression in plants?

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What is the minimum number of cells required for fluorescence in situ hybridization?

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Single-cell cloning from SkBr3?

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What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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MiRNA qpcr problem?

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Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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Arvind Singh

Please check these useful links and pdf attachments for the said query.

http://www.sciencedirect.com/science/article/pii/S0012160605007645

http://www.nature.com/articles/srep21577

https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-3-12

Goof Luck

Please check these links also.

https://www.researchgate.net/post/What_is_the_best_way_to_overexpress_miRNAs

http://jxb.oxfordjournals.org/content/61/9/2271.long

http://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-015-0413-9

http://www.protocol-online.org/biology-forums-2/microRNA.html

http://journal.frontiersin.org/article/10.3389/fpls.2015.00099/full

https://www.researchgate.net/publication/268380733_Advance_On_Detect_Methods_of_Plant_Microrna_Expression

Article Advance On Detect Methods of Plant Microrna Expression

Soumi Biswas

@Arvind Singh Thank you very much.