The cells were grown in a DMEM/F-12 media with 5% FBS and incubated at 37oC in 5% CO2
What about ph meters or indicator papers??
I am not very sure about what do you mean with "decanted from a cell culture".
If you want to measure the medium PH, you have different PH-meters (digitals, paper-based...)
Please specify in more detail your trouble or question, and maybe I could be more helpful.
Best regards,
Jose María.
Decanted means discarded. In other words: the old and used medium, which should be very acidic.
all conventional methods are suitable.
you just pay attention in measuring as soon as you decant, because temperature and/or air fastly imbalance the pH of the medium.
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