Our lab is working with herbal plants and phytochemical analysis. On internet resources I did not find any specific literature. Can anyone help me.
You need to be specific to the group chemistry!
For example
flavonoids in general have typical UV spectra
anthocyanins have typical UV-Vis spectra
furastanol and other steroidal saponins - derivatisation and spectroscopic assays are available.
If you know what groups of compounds you are looking for, then it is possible to direct to the correct literature
Spectrophotometer can be used to detect the phytochemical constituents qualitatively as well as quantitatively. In qualitative approach you will only be able to know about the presence and absence of certain phytochemical in your herbal specimen. While following the quantitative approach one can know about the amount of certain phytochemical in respective sample. But, for precision you may have to follow the chromatographic techniques like HPLC etc.
You can do HPLC, HPTLC profilng by comparing with standard phytomolecules. Or else you can go with GC-MS or LC-Ms to find out probable phytochemical present in the herbal extract.
HPLC-MS is suitable for most phytochemicals. GC-MS for volatiles e.g. essential oils, fatty acids and acid esters, etc.
Agree With Rajesh, HPTLC is relatively simple to determine of phytochemical compound on plant sample.
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