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Questions related from Jovale Vincent Tongco
I am looking for clear-cut protocols for the quantification of tyrosinase inhibition of plant extracts or isolated compounds from plant sources. What are the differences if crude extracts were...
26 March 2014 4,484 4 View
I may have detected vitamin D3 from my plant extracts using GC-MS. I searched the literature and came up with studies that say it's possible and others that says otherwise. What is your expert...
25 February 2014 5,194 10 View
Does anyone know a free online UV-Visible spectra database where someone can compare and verify their experimental spectra with? I am dealing with organic compounds (e.g. phenolics).
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Can anyone provide some relevant information on using column chromatography in separating phenolics from crude plant extracts? Is it ok to use silica gel or do I have to use another type of...
26 September 2013 2,073 24 View
I want to test the ACE inhibitory effect of my phenolic extracts. Can you give me a proper and suitable protocol for doing it? My phenolics came from the leaves of a plant material.
12 July 2013 4,919 9 View
I'm thinking of using either 2D paper chromatography or column chromatography. Which do you think is better if I have many compounds on my sample and I want to estimate their quantity?
02 June 2013 1,432 6 View
I am planning to use either DPPH, ABTS, Nitric Oxide, or Ascorbic acid assay. Due to financial constraints, I can only do two of these assays. Can you help me weigh down my options? Other methods...
17 May 2013 2,064 10 View
What solvent (system) would you recommend if I'm to extract major components from a commercial tea product. I am thinking of using acetone-water as my solvent. I am planning to do Soxhlet...
29 April 2013 9,243 8 View