What is the difference between variation in displacement and virtual displacement?

Also when referring to variation in the work (virtual work), we use virtual rotation not rotation variation? Thanks.

05 April 2019 3,332 10 View

Could I model a reinforced concrete member as a wire element in ABAQUS?

In shell element I could define rebars layers to define reinforcement, but could I implement rebars in wire element? Thanks

26 March 2019 2,223 8 View

How to implement plastic hinge with capacity interaction between axial load and bending moment in ABAQUS?

In 3D frame analysis, using elastic element with plastic hinge at nodes accelerate the solution time instead of using distributed plasticity. But most plastic hinges include interaction between...

26 March 2019 2,450 3 View

What is the allowable lateral acceleration of tall building due to wind load?

People feel uncomfortable in the upper stairs of the tall building when the building move laterally due to wind load or to be accurate the lateral acceleration of the building. Is their any...

21 March 2019 9,055 9 View

At implementing damage model, How could I get the effective stress-strain curve or damage parameter from true stress strain curve?

Most experimental results include true strain curve. How could I experimentally or theoretically reach effective stress strain curve or damage parameter for damage model? Thanks

01 March 2019 9,017 11 View

Does anyone read about using multi-directional cracks in 3D modeling of concrete?

I encountered using multi-directional cracks model in 2D elements, but could it be used for 3D concrete elements?

23 February 2019 384 14 View

What is the shape of loading and unloading curve of concrete in tension?

Also how could I identify the pre-transition and post-transition parts of strain increment?

21 February 2019 1,135 5 View

Do anyone have a simplified explanation, reference, or source code in using a return-map algorithm in plasticity?

I encountered a return-map technique in many papers but I do not understand how it works.

14 February 2019 8,995 3 View

Why does Etabs software calculate magnified moment (ACI -318) due second order effects in a different way?

These attached figures are captured from Concrete Frame Design Manual using ACI-318 attached with Etabs Design examples. It calculates the amplified moment due to second order effects using this...

14 February 2019 6,986 3 View

Why does arc-length method diverge when the stiffness approach zero?

10 February 2019 6,214 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View