Can anyone suggest a protocol to study immunomodulatory activity of mesenchymal stem cells?

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How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What happen if a transcription factor bind directly to not only the regulatory region but also the target gene sequence?

As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...

13 May 2024 1,099 3 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

Can I use a different grade of cells for my positive control?

I am performing PCR as a QC test to look for a transcription gene that should be negative after a CAR T therapy process. As we are comparing against a CAR transduced patient's cell, we require a...

22 April 2024 5,262 1 View

How do you remove snow particles on cryoboxes that were stored in LN2?

I have difficulty on removing snow particles on cryoboxes, storing cryovials. The snow particles can sometimes take a lot of space and causing difficulty in closing the cryobox which will later be...

19 February 2024 6,690 0 View

Can I merge T cell from two different host?

My lab co-worker need a lot of T cell for his animal experiment, however one of the donor can't provide enough for downstream analyses, we thought to combine purified T cells from two donors in...

06 November 2023 7,188 0 View

Does the computer system architecture of RFID toll system in Malaysia uses pipelining? Where can I find information about it?

I was told to propose a computer system architecture design to overcome the problem of RFID toll system and compare the proposed design and existing design about the implementation of pipeline.

25 July 2023 2,961 0 View

Are there some new-discovered exposures in the context of gestational diabetes mellitus?

Especially related to nutritions.

14 July 2023 1,072 3 View

Cytoscape loading node error?

I was trying to use Cytoscape to replace IPA to fit our labs convenience, also with no self-generated database or profiles I feel the need to start with the Biogateway app, however whenever I...

03 July 2023 9,412 0 View

What cause the Gloo after incubation using Qiagen DNA extraction kit?

I was performing the DNA extraction of cells with a concentration of 5x10^6. After adding AL buffer and proteinase K an incubation was done. After the incubation period at 56 degree Celsius, I...

27 June 2023 9,761 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

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05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

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How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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Amera Khalil Mohammed

You can use it in two groups of animals, one group use immunosuprssion and the other one non immunosupressed ,then compare the two groups for immune reaction

Dean Phillip John Kavanagh

A number of people use T-cell supression assays; they are relatively easy to do in vitro and pretty robust. Have a look at some of these papers: http://www.ncbi.nlm.nih.gov/pubmed/15963858; http://www.ncbi.nlm.nih.gov/pubmed/19565371

Raja Sundari Meenakshi Sundaram

You can co-culture the MSCs with Pheripheral blood mononuclear cells and stimulate the proliferation by adding mitogen like phytohaemagglutinin. Lymphoblast acquistion can be done by flow cytometry.. Various cytokines produced can be quantified by ELISA ..