I have difficulty on removing snow particles on cryoboxes, storing cryovials. The snow particles can sometimes take a lot of space and causing difficulty in closing the cryobox which will later be stored in the LN tank.
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View
Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...
14 February 2021 2,039 4 View
My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...
10 February 2021 3,580 1 View
I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...
09 February 2021 9,012 3 View
I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...
04 February 2021 5,925 9 View
I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...
04 February 2021 6,580 2 View
I want to simulate a uniform stress condition in the vertical direction on the surface of a spherical particle. Since it is very difficult to describe the boundary conditions of a complete...
31 January 2021 2,726 1 View
Surely they will want to avoid contact with air/moisture, so what is the procedure for this?
23 January 2021 428 2 View
Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....
02 March 2021 3,053 2 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...
28 February 2021 6,282 9 View
In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...
26 February 2021 2,435 4 View
Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...
25 February 2021 7,493 6 View
I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...
20 February 2021 199 2 View
I am looking for literature suggestions on the topic of snow aggregation. My main interest is on the aggregation process itself (rather than papers describing aggregate snowflakes characteristics)...
19 February 2021 2,197 3 View
I don't know much about molecular dynamics, but I am suspicious of my pressure being equal to kinetic energy. I am using // sum the velocities squared: double v2sum = 0.0; for (int i = 0; i
15 February 2021 1,316 3 View
Recently, I've been selected for an ICTP program named Physics of Complex Systems. But, I have a keen interest in Particle Physics & Quantum networks. As statistical mechanics involved in...
11 February 2021 699 3 View
De Broglie's double-solution postulated a causal model of Quantum Mechanics. Knowledge of the photon structure was needed to model the particle part of the cause of the Double Slit interference...
11 February 2021 6,869 5 View