What are the factors we should keep in mind while submitting the article to journal (*general question)?

What are the factors we should keep in mind while submitting the article to journal? Why many papers get rejected even though they contain proper content? Why few journals take years of time...

01 February 2021 3,130 2 View

Can anyone please explain about the renaturation of two different subunit of recombinant protein?

Thanks in advance to everyone who answers my question. I want to raise a recombinant N-methylhydantoinase protein by pET expression vector system. The protein is a tetramer of two identical small...

18 January 2021 1,026 2 View

How the journal article handling time varies?

Some of my papers are still under review since 11 months. But when I checked journal home page the accepted articles received dates are varying, some are received 2 months ago and some are...

04 July 2020 3,209 3 View

Improving STEM Education with Computer Based Learning?

Dear Colleagues, For an article we are writing, we are looking for good references (to be cited) that cover topics about using computers and artificial intelligence in STEM learning. Do you have...

30 April 2020 9,709 14 View

Ever used an Intelligent Tutor?

Have you ever used an intelligent tutor in your classroom? If yes, please share your experiences. How did you like it? How did your students like it? Do you think the students actually learned...

05 April 2020 2,356 17 View

How do you estimate required sample size in comparison of a diagnostic test against gold standard with ordinal categorical outcome?

I am currently designing a trial to evaluate the performance of a diagnostic test by comparing it against an established standard. The outcomes are ordinal in nature, integer values ranging from 0...

27 August 2019 7,601 3 View

I want to run a linear trait such as BMI in GWAS. How to determine which all covariates need to be adjusted and is there an option in SNP test?

I want to run a linear trait such as BMI in GWAS. How to determine which all covariates need to be adjusted and is there an option in SNP test?

16 July 2019 5,723 2 View

What is the chemistry behind low and high H - termination in High purity Germanium ?

During fabrication of HPGe for detectors we are doing low and high H-termination. What is the chemical reaction behind this?

15 June 2019 9,944 1 View

What are the basic things to consider while initiating a CRISPR based genome editing?

Is it necessary to have whole genome sequence to study data CRISPR based gene expression in bacteria? what are the other requirements to initiate the process.

18 February 2019 9,540 2 View

How to solve abaqus MPI error while trying to restart an explicit analysis ?

Hello all, I am running a explicit analysis with two steps, and I have provided restart command to both the steps so that data gets saved at every x intervals. The simulation stops after the...

04 November 2017 9,988 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View