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Questions related from Aravind Tallam
I have been working with Human primary macrophages (differentiated from monocytes isolated from blood) and the experiments done to date with siRNA and shRNA (Nucleofection) in these cells were...
29 November 2013 2,870 0 View
I have differentially expressed genes from my microarray data and i would like to know if there are any knowledge sources/tools with the collection of all known Transcription factors from...
03 September 2013 8,046 6 View
I have a network with 500 nodes and 400 edges. Iam interested in the pathways between 2 nodes and for that i have calculated all the shortest paths between that 2 nodes. To test experimentally, i...
30 August 2013 506 4 View
Using GEO data and network inference methods (GRN), I have got relationships between genes from each GRN method (example, GENIE3, CLR, LASSO). For specific genes which I am interested, I have got...
21 March 2013 6,006 3 View
I downloaded a dataset from GEO (GSE1906) and they have only 1 sample (I mean with out any replicates/triplicates) per condition and their data files contain the measure of the abundance of a...
21 March 2013 2,353 5 View
As you might know that some of the datasets in GEO will contain the data in SOFT formatted files instead of CEL files which contains the abundance of the transcripts. Do we need to consider these...
21 February 2013 7,281 8 View
For the differentiation of human primary monocytes to macrophages, I use human serum added to RPMI. Due to some problems with the human serum, the cells isolated from a lot of blood samples...
15 October 2012 9,601 11 View
I will be sacrificing mice and will extract blood by cardiac puncture. I need to isolate monocytes and differentiate them into macrophages. I found some papers but they modify the protocol...
01 March 2012 2,955 12 View
