Can anyone suggest a mlst (multilocus sequence typing) protocol of wolbachia ?

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DdPCR analysis: biological replicates ?

--Hi, such as RTqPCR to analyse gene expression, is it necessary to have biological replicates in a ddPCR experiment ? thank yu --

21 May 2024 8,181 6 View

Model for reconstructing the number of inhabitants from the number of burials ?

hi, I'm trying to find out if there are any models for the medieval period south of the Sahara in West Africa that can be used to reconstitute the number of inhabitants at a site from the number...

04 November 2023 2,666 1 View

Aerosol biocontamination and sanitation network ?

Good morning, I am looking for publications/data regarding aerosol biocontamination from the sanitation network lift stations. Would you be aware of any articles or complications on this...

17 July 2023 2,369 0 View

Freeze Drying small volumes : What should be considered ?

Hi All, we usually discuss different tips in order to freeze dry large volume samples (e.g. two steps freezing, etc). I would like to ask you if you have any tips regarding the small volumes ? For...

20 June 2023 4,324 2 View

What do we know about the costs of synthetic fuels in road transport?

From 2035 on, besides fully electric cars, the EU will also allow the putting on the market of cars powered by synthetic fuels/e-fuels. Do you know an up-to-date, reliable independent estimate of...

29 March 2023 9,160 2 View

What is the significance difference between a p-value of 0.01 and a p-value of 0.012 ?

--Hi, p-value = 0.01 and p-value = 0.012 is it risky to say that it is the same thing in terms of probability ? p=0.01 --> * * p=0.012 (p>0.01) --> * Thank you --

09 November 2022 7,448 3 View

How to sample blood on X. laevis ?

Hi everyone, I am currently working with X. laevis and I would like to collect blood samples. This would happened right before euthanasia on anesthetized individuals. My aim is to measure...

20 June 2022 8,691 0 View

Could you recommend a method or pipeline for quality control and paralog removal from amplicon sequencing data?

Hello everyone, I have recently started working with NGS data (amplicon sequencing) for phylogenetics and species-delimitation studies of closely-related specimens (typically within a genus or a...

02 April 2022 3,139 0 View

Are changes significant?

Dear everyone, I've seen many posts in this community that seem to answer my questions but I have a tough time figuring out how my situation compares to the one described by the op's and I'm...

24 December 2021 7,852 4 View

Who can tell me how to employ an Endogenous-Switching Poisson regression model ?

I want to employ Endogenous-Switching Poisson regression model to determine the impact of x on Y. However, I don't know the succession of the variables from to the command , dependent variables ,...

06 September 2021 8,159 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View