It seems very difficult to find a good sertoli cell line to work with, any suggestions?
Thanks. I am currently trying to obtain a sertoli primary culture......
Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...
29 July 2024 3,733 5 View
Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...
18 July 2024 382 5 View
Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...
10 July 2024 8,880 4 View
Hello to everyone, I am trying to launch a BLYP optimization with ORCA starting from an already converged calculation at BP level ,exploiting its orbitals and geometry as a guess. I tried...
24 June 2024 3,768 2 View
A binary classifier based on multivariate Gaussian models, which estimates the mean vector and the variance-covariance matrix during the training phase and returns the class with the highest...
23 June 2024 10,114 1 View
Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco
09 May 2024 2,070 11 View
Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...
24 April 2024 6,359 4 View
What should we do with high-correlation data? Did we remove them, or do they not matter? WHY?
12 April 2024 9,429 3 View
Greetings. In the context of the new ISO 14068 on Carbon Neutrality, how is double-counting of emissions between two subjects been dealt with? Suppose Company B is assessing the carbon neutrality...
03 April 2024 3,596 1 View
Is there any standardised index to estimate, more or less precisely, muscle mass from body weight (e.g., Bodyweight * index)? Any reading suggestions are much appreciated.
27 February 2024 8,285 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View