Can anybody tell me about a pharmaceutical laboratory of UK?

06 July 2015 3,523 0 View

What happens if we expose high glucose level to RBC in vitro? Does it damages the RBCs?

Can high glucose only effect RBC?

07 August 2013 8,331 2 View

What is the method by which we can introduce drugs, plant extract to a streptozotocin induced rats?

I treated the rats with plant extracts (in a specific concentration) but am not getting the expected result. I have dissolved the plant extracts with their drinking water but still not got any...

03 April 2013 2,621 9 View

Can anyone suggest about a technique by which I can rapidly screen for the medicinal plants having antidiabetic activity?

Inducing a rat with streptozotocin is a very long procedure. After giving streptozotocin in proper dose and maintaining all the conditions, I get no result, the blood glucose remains normal after...

31 December 2012 8,321 3 View

Does streptozotocin only effect the pancreas?

Streptozotocin is a drug, which is also a carcinogen. Besides the pancreas, can it harm other vital organs? Are there any results related to this topic?

09 October 2012 4,328 4 View

Can a single dose of Streptozotocin create a diabetic rat?

I have injected the drug (45 mg/kg body wt) into two rats. After 3 days of streptozotocin treatment the blood glucose level increased but when I tested the glucose level repeatedly at 2-3 days of...

06 July 2012 9,730 1 View

Is there any protocol by which we can estimate glucose of bacteria?

We can estimate the blood glucose ,starch content of plants,like that is there any methods by which we can estimate the glucose content of bacteria?Actually I want to know about the mode of...

01 February 2012 7,340 12 View

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the...

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the strain among them produce pink pigments,the...

01 February 2012 7,188 0 View

What actual strain of mice can I use for diabetic model?

Actually I am trying to induce diabetes in rats but the result is not satisfactory. Because I am getting no results. the FBGL does not exceed beyond 126mg/dl. Now I am trying the same thing in...

01 January 1970 322 3 View

Can any one tell me the actual doses of Alloxan or streptozotocin to induce diabetes?

I am working on the antidiabetic activity of plant extract

01 January 1970 928 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

The lipid bilayer broken down and form micelle within 1ns MD simulation. How to simulate lipid bilayer without breaking bilayer?

To simulate the lipid bilayer (10-10, 25-25 so on) from CHARMM-GUI membrane builder, we used to simulate it at various force field (AMBER14, AMBER03, AMBER14IPQ, AMBER15FB, NOVA, YAMBER) at water...

26 January 2021 7,816 2 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

SEM/TEM/AFM which analysis should be performed for spray dried solid lipid nanoparticles?

Can anybody please suggest which analytical technique would give me the best result for the size and shape of solid lipid nanoparticles SEM or TEM or AFM. The nanoparticles are synthesized by...

21 January 2021 7,991 6 View

What is role of chronopharmacology or circadian rhythms in metabolic diseases including obesity, diabetes and cardiovascular diseases?

Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...

18 January 2021 9,045 3 View

Why the lipid accumulation levels in my 3T3L1 does not match with my adipogenic and lipogenic gene expression levels?

I am using the 3T3-L1 cell line to discover the adipogenic effects of a natural compound and its derivatives. I conducted a lipid accumulation assay using fluorescent Nile Red dye (Adipored) for...

10 January 2021 4,360 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

What type of interventional study is this?

Hi, I'm evaluating some medical papers to write a review, but I'm having trouble defining the study design of the following paper: Neurophysiological recovery after carpal tunnel release in...

04 January 2021 1,571 4 View