Can anyone suggest a good protocol for the estimation of lipid peroxidation?

Can anyone suggest a good protocol for the estimation of lipid peroxidation?

07 August 2013 4,832 5 View

What happens if we expose high glucose level to RBC in vitro? Does it damages the RBCs?

Can high glucose only effect RBC?

07 August 2013 8,331 2 View

What is the method by which we can introduce drugs, plant extract to a streptozotocin induced rats?

I treated the rats with plant extracts (in a specific concentration) but am not getting the expected result. I have dissolved the plant extracts with their drinking water but still not got any...

03 April 2013 2,621 9 View

Can anyone suggest about a technique by which I can rapidly screen for the medicinal plants having antidiabetic activity?

Inducing a rat with streptozotocin is a very long procedure. After giving streptozotocin in proper dose and maintaining all the conditions, I get no result, the blood glucose remains normal after...

31 December 2012 8,321 3 View

Does streptozotocin only effect the pancreas?

Streptozotocin is a drug, which is also a carcinogen. Besides the pancreas, can it harm other vital organs? Are there any results related to this topic?

09 October 2012 4,328 4 View

Can a single dose of Streptozotocin create a diabetic rat?

I have injected the drug (45 mg/kg body wt) into two rats. After 3 days of streptozotocin treatment the blood glucose level increased but when I tested the glucose level repeatedly at 2-3 days of...

06 July 2012 9,730 1 View

Is there any protocol by which we can estimate glucose of bacteria?

We can estimate the blood glucose ,starch content of plants,like that is there any methods by which we can estimate the glucose content of bacteria?Actually I want to know about the mode of...

01 February 2012 7,340 12 View

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the...

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the strain among them produce pink pigments,the...

01 February 2012 7,188 0 View

What actual strain of mice can I use for diabetic model?

Actually I am trying to induce diabetes in rats but the result is not satisfactory. Because I am getting no results. the FBGL does not exceed beyond 126mg/dl. Now I am trying the same thing in...

01 January 1970 322 3 View

Can any one tell me the actual doses of Alloxan or streptozotocin to induce diabetes?

I am working on the antidiabetic activity of plant extract

01 January 1970 928 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Error in comsol?

Dear respected researchers, while performing CFD analysis in Comsol it's showing like this Error: Nonlinear solver did not converge. The maximum number of Newton iterations reached can...

24 February 2021 249 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View