Can anybody tell me about a pharmaceutical laboratory of UK?

06 July 2015 3,523 0 View

Can anyone suggest a good protocol for the estimation of lipid peroxidation?

Can anyone suggest a good protocol for the estimation of lipid peroxidation?

07 August 2013 4,832 5 View

What is the method by which we can introduce drugs, plant extract to a streptozotocin induced rats?

I treated the rats with plant extracts (in a specific concentration) but am not getting the expected result. I have dissolved the plant extracts with their drinking water but still not got any...

03 April 2013 2,621 9 View

Can anyone suggest about a technique by which I can rapidly screen for the medicinal plants having antidiabetic activity?

Inducing a rat with streptozotocin is a very long procedure. After giving streptozotocin in proper dose and maintaining all the conditions, I get no result, the blood glucose remains normal after...

31 December 2012 8,321 3 View

Does streptozotocin only effect the pancreas?

Streptozotocin is a drug, which is also a carcinogen. Besides the pancreas, can it harm other vital organs? Are there any results related to this topic?

09 October 2012 4,328 4 View

Can a single dose of Streptozotocin create a diabetic rat?

I have injected the drug (45 mg/kg body wt) into two rats. After 3 days of streptozotocin treatment the blood glucose level increased but when I tested the glucose level repeatedly at 2-3 days of...

06 July 2012 9,730 1 View

Is there any protocol by which we can estimate glucose of bacteria?

We can estimate the blood glucose ,starch content of plants,like that is there any methods by which we can estimate the glucose content of bacteria?Actually I want to know about the mode of...

01 February 2012 7,340 12 View

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the...

Recently ,I have isolated Agrobacterium from tumor on potatoes(potato disc assay).But along with the agrobacterium I get some other strains.One of the strain among them produce pink pigments,the...

01 February 2012 7,188 0 View

What actual strain of mice can I use for diabetic model?

Actually I am trying to induce diabetes in rats but the result is not satisfactory. Because I am getting no results. the FBGL does not exceed beyond 126mg/dl. Now I am trying the same thing in...

01 January 1970 322 3 View

Can any one tell me the actual doses of Alloxan or streptozotocin to induce diabetes?

I am working on the antidiabetic activity of plant extract

01 January 1970 928 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

What is role of chronopharmacology or circadian rhythms in metabolic diseases including obesity, diabetes and cardiovascular diseases?

Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...

18 January 2021 9,045 3 View

How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View