i also require same protocol

This article provides a lot of useful information on how to measure glutathion-associated enzymes in plants:

http://scialert.net/fulltext/?doi=ajpp.2007.195.205

Regards, Christian

You can perform standard assay methods for GR and GPX enzymes after extraction of plant sample with100mM Tris -HCl buffer pH 7.5 and 1mM EDTA the centrifugation for 15 min  at15,000xg use the supernatant as the source of enzyme performed assay protocol as described see Hunaiti etal1995 Papers are in my research gate Good luck

Dear Christian...Your paper is not going to open.Please email me.My id is [email protected]

Glutathione reductase (GR; EC 1.6.4.2) activity can be assayed as described by Carlberg and Mannervik, 1975, by measuring the oxidation of NADPH at 340 nm. The reaction mixture consisted of phosphate buffer (0.1 M, pH 7.5), 50 mM MgCl2, 16 M oxidized glutathione (GSSG), and 8 mM NADPH and was initiated by addition of supernatant. The activity of GR should be calculated based on tissue protein concentration and expressed as relative enzyme activity. I hope this will serve your purpose.

@Samrina: I sent the paper to you.

Cheers, Christian

thanks Christian

Good question with great answers.

Thanks

Thanks all for your valuable reply.

I have extracted flavonoids from plants. Can I check glutathione peroxidase and catalase activity in my extract? or I should check these activities during stress conditions? Abdelrahim Ahmad Hunaiti

Dear Rakhshan

you can assay glutathione peroxidase and catalase in crude extracts under stress conditions or under normal condition provided you used the standard assay protocols and you can com pair the activities

Thanks alot @abdelrahimahmadhunaiti

Protocols for measuring SOD, CAT, GPx, GR, GST, GSH and SH groups

https://www.researchgate.net/publication/340429895_Supplementary_material_manuscriptv7docx

If you have any questions write.