Can Anyone share research on age of diagnosis of Type 1 diabetes?

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How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

What is your view point regarding the edible coating on the green leafy vegetables?

1. Nutritional contribution of the edible coating 2. Self life of the vegetable to be coated with the edible coating. 3. Toxicity development after preserving the vegetable with the edible coating.

11 June 2024 1,740 3 View

During ws2 growth via cvd I have noticed black round spots on the si(100) substrate. please suggest me reason behind this?

I am using 20 mg of WO3, 800 mg of Sulphur, 925 oc growth temp, 280 oc S temp, and a 20 sccm gas flow rate.

05 May 2024 8,665 2 View

Can I allow cell phones in SPF animal house?

I have questions regarding the safe use of cell phones in animal research facilities. While I acknowledge the hygienic risk, we often spend a lot of time inside our barrier SPF facility and we...

14 April 2024 3,486 0 View

Inquiry Regarding Research on Female Sex Tourism in Jamaica?

Ladies and Gentleman, My name is Jana Maric, and I am a tourism management student at the university of applied sciences in Germany currently researching for my bachelor´s thesis in the field of...

08 April 2024 4,449 0 View

Can you find this relation ((β²+α²)/2αβ)sinh βb sin αa + cosh βb cos αa = cos k(a+b) from given determinant?

Please do it by hand, if not possible just do by some analytic engine.

26 March 2024 2,028 0 View

Why did I get the cracks on thin film after annealing? And how can I overcome this problem?

I deposited Ga2O3 thin film on fused quartz substrate at 500oc in Ar ambient by RF sputtering and then annealed at 900oc (very slow heating and very slow cooling i.e 1oc/min) in air ambient for 1...

11 February 2024 3,417 4 View

How to incorporate the negative slope (necking) part of an experimental tensile test data in ANSYS?

ANSYS multilinear material model does not support plastic strain vs stress data with a negative slope. Moreover, it assumes perfectly plastic (horizontal) behaviour from the end data point. How...

01 February 2024 9,950 0 View

Regarding UGC care list journal?

INDIAN JOURNAL OF NATURAL SCIENCES ISSN: 0976-0997 ( Print, Bimonthly ) Multidisciplinary Sciences Web of Science Indexes: Zoological Record is this journal UGC care list II category?

25 December 2023 4,017 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

THP-1 differential markers (M0, M1 and M2). what do you recommend?

Hello, what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings

24 June 2024 9,025 0 View

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

How to mimic diabetic conditions in cell culture?

Is there a protocol to establish diabetic conditions in pre-osteoblasts cell culture?

18 June 2024 9,306 2 View

How does the presence of diabetes affect the prognosis of myocardial infarction?

Comprehensive Overview of Myocardial Infarction: Etiology, E... How does the presence of diabetes affect the prognosis of myocardial infarction?

15 June 2024 1,038 3 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View