Steroid extraction
Any kind of steroids can be extracted using Harbourne Protocol [Phytochemical Methods, check out this book online or in your local library], usually a chloroform-methanol mixture helps !
Hello, currently, I am working on the chloroplast genome and experiencing difficulties in reading ambiguous code N in the intron. I have blasted it to NCBI, but there are no nucleotides aligned...
02 July 2023 1,703 0 View
I want to extract lineament using Sentinel-1 data but I still confused to choose between SLC or GRDH data. Beside that I still confused about what polarization that fits to do extraction.
11 October 2022 6,951 0 View
I am interested in conducting a sociolinguistic research on TikTok videos. I need your views on its relation to Stuart Hall's theory of representation.
18 May 2020 8,403 0 View
Some methods described how we can analyze the anti hematin formation from compounds isolated from fungal endophytes. But can we directly add the beta-hematin to the culture so the fungi start...
16 November 2015 7,027 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View