Can anyone recommend an appropriate vibration criteria for IVF clinics in addition to the VC-A/VC-B criteria for optical equipment?

More Francesca Remigi's questions See All

How do you collect the celiac ganglion from mice?

Does anyone know how to / anatomical landmarks to collect the celiac ganglion?

06 December 2023 8,709 0 View

Can you tell me a protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

Hello everyone, Can you tell me a standardized protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

14 November 2023 1,546 1 View

Freeman Tukey test: is there an online tool?

Hi, I have data in form of counts (number) and I wanted to run a statistical test to compare the counts between groups to see if there is statistically significant difference between groups. I...

24 October 2023 1,545 3 View

How can I prevent exosomes from clumping together in PBS for NTA after isolation?

I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...

13 August 2023 4,773 0 View

Has anyone had problems with the PyroMark Q48 cartridges?

Hi everyone, a few months ago we bought the PyroMark Q48 to replace the old Q96, but we used it very little due to continuous malfunctions of the cartridge with enzyme and substrate. We've already...

23 July 2023 8,854 0 View

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices?

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices? Can someone provide a study on the dose-response relationship of glutamate in brain slice preparations?

11 July 2023 9,547 1 View

At which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

at which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

13 June 2023 7,880 2 View

Is it possible to quantify 16S regions in a sample of WGS data?

Hi, I would like to know if there is any software readily available which allows the identification and quantification for 16S regions in WGS data. Thanks n advance for any advice/help :)

15 May 2023 1,177 0 View

How do you estimate parts of the biofilm being sediments or dead organic material of biomass collected from river stones?

We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...

27 March 2023 2,413 1 View

I keep encountering this error when trying the Cast transects in DSAS?

--------------------------- DSAS Error --------------------------- An unexpected error occurred. Please refer to the log file (C:\Users\Deighl\AppData\Roaming\Corona Environmental Consulting\DSAS...

23 January 2023 7,611 0 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View

What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?

02 July 2024 9,204 0 View

In a post-harvest handling chain (harvesting, transportation, storage), which among these do you think has the highest percentage loss why?

02 July 2024 7,649 0 View

Are there recent publications on the economic comparison of Solar PV Curtailment vs. BESS Installation?

I'm currently exploring the economic implications of solar PV curtailment versus the installation of battery energy storage systems (BESS). Specifically, I'm interested in recent publications or...

27 June 2024 6,423 2 View

Effectiveness and Implementation of CCUS in Oil Fields: Genuine Progress or Demonstrative Effort?

Many studies have been conducted on Carbon Capture, Utilization, and Storage (CCUS) in oil fields to increase the recovery factor. Do you believe these studies are genuinely useful, or are they...

24 June 2024 7,441 0 View