Samantha Rustichelli

3 Questions 1 Answers 0 Followers

Questions related from Samantha Rustichelli

Can anyone recommend a protocol for mESCs FA1% fixation in solution for ChIP ?

Hi, in the protocol we have in the lab we always fix mECS by adding 1% FA solution (diluted in PBS) directly on cells attached to the plate. We leave it 10mins RT with gentle shaking and we quench...

22 January 2024 785 0 View

How to extract nuclei from cultured neurons?

Hello, I am currently differentiating mESCs into neurons. I would like to perform a subcellular fractionation, followed by nuclei lysis and IP. Does anyone have a protocol to suggest? I have never...

22 November 2023 7,456 0 View

Does Anyone Recommend a Protocol for the Cell Cycle Synchronization of mESCs ?

Hello everyone, I would like to synchronise mouse embryonic stem cells in either G1 or G2/M phase in order to study the transcription of a set of genes of interest. I'm having some trouble finding...

26 May 2022 2,531 1 View