Hello all,
I want to express miRNA candidates in the PC cell line, and I would like to know what is the best protocol for cell numbers and the miRNA conc.
Thanks for the information.
Dear Alia:
Please check the following journal articles:
Effects of dietary flavonoids, luteolin, and quercetin on the reversal of epithelial-mesenchymal transition in A431 epidermal cancer cells.
Cancer Science 07/2011; 102(10):1829-39. DOI: 10.1111/j.1349-7006.2011.02035.x
Source: PubMed.
Impact of Flavonoids on Matrix Metalloproteinase Secretion and Invadopodia Formation in Highly Invasive A431-III Cancer Cells.
PLoS ONE . 01/2013; 8(8):e71903. DOI: 10.1371/journal.pone.0071903
Source: PubMed
Best of luck!
check ibidi.com
Dear Alia,
Not easy to respond to your "a little big vague" question.
Whatever it could be, the two attached documents may be of help.
Best regards
Robert
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hello Dear All I am a new user of VASP (Vienna ab-initio simulation packages) and tried to calculate the phonon using VASP. I get the phonon band structure (after calculations) in image form(.png...
11 December 2023 9,204 6 View
Background: I am working on checking the in-vitro immunogenicity of a molecule encapsulated in a PLGA (microspheres) drug delivery system. After the addition of the drug to the cells, I observed...
12 July 2023 6,139 0 View
Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...
17 April 2023 3,999 0 View
I have already processed my fish gut samples for microplastics analysis from extraction to filtration and drying. The extracted microplastic particles were stored in dried glass fiber membranes....
16 April 2023 8,457 2 View
How to prepare a stock solution of α-Amylase from Porcine Pancreatic powder from Megazyme with an activity comparable to a 1000U/mg stock solution of α-Amylase from Porcine Pancreas from...
29 March 2023 3,397 0 View
Is there a software that can represent the hcp crystallographic orientation, and give a quantitative analysis of these orientations given an EBSD picture?
18 March 2023 3,753 0 View
The layered materials, also called the van der Waals (vdWs) materials, are those having strong in-plane bonding and weak vdWs interactions in out-of-plane directions (e.g. graphene or h-BN). The...
01 February 2023 8,315 2 View
How to conduct a systematic literature review (SLR)? What sould it include? Could you share resources including guidlines for writing an SLR? What is the difference between an SLR and a regular...
03 January 2023 10,024 4 View
I want to build a song recommendation engine based on the difficulty of singing a song. I am unable to find any prior research or method of evaluation for a song. Help much appreciated.
28 November 2022 1,892 13 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View