I am looking for a working CD105 AB for IF in mice. I tried one from Millipore but it did not work for me.
I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...
05 June 2018 1,245 6 View
Part of the retrospective evaluation of a pathology "XY" is the question "Since when did you realize those symptoms?". As answers I get a variety of answers, like: - "since 6 days - since 2-3...
10 November 2017 2,787 5 View
I want to analyze the results of a plate with a target gene and 2 or 3 housekeeping genes. How does it work? How to combine the housekeeping genes? How would the calculation (delta Ct; delta-delta...
11 December 2013 6,648 21 View
I calculated it myself, with dilution calculators (Sigma) and let colleagues do it - everybody gets a different result. Can you help me? - Substance X - Molecular Weight 300 - Dilution for...
07 August 2013 1,034 2 View
I would like to mix the differentiation-medium (adipogenesis/osteogenesis) for murine MSC myself. I found several publications about this, basically it comes down to: Basal medium + FCS +...
06 July 2013 5,500 6 View
I would like to try to acellularize a rats liver. How to do it? What medium do you use for the perfusion? What max. pressure do you use?
06 July 2013 8,262 6 View
There are several different methods described for CFU-F assays for MSC. E.g. in the book "Mesenchymal Stem Cell Assays and Applications" there are several partly very different methods described....
06 July 2013 9,888 5 View
I wonder if it is possible to dissociate glandular tissue by an agent like collagenase / accutase etc. and do an flow cytometry / cell sorting right afterwards with those cells. Or do you think...
06 July 2013 6,244 9 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View