I'm sure that mechanical pressure will acellularize the liver or maybe even using a sonicator for cell separation by sonoporation.

It has been known that even acetic and PAA help decellularize tissues while maintaining or retaining the ECM in place.

Regards

Charan

Hi Roth

I think the efficiency of the acellularization methods dependend on the tissue type and the acellularoization methods used. The currently used effective acellularization involvesNaCl-SDS, TritonX-100 and Enzymatic method. The nature of substance and time of acellularization process effects both physiology and morphology of the tissue.

There are many published papers on Heart acellularization in PubMed. Hope this helps you.

thanks

RKG

I would strongly suggest to check this article: Soto-Gutierrez et al., Tissue Engineering: part C 2011;17(6):1-10. Of course the nature of the tissue strongly important. are we talking about human or animal tissue? if human, keep in mind that steatosis and fibrosis level are strongly important for your outcome.

Thanks everybody!

1. portal perfusion with SDS

Reference: Bryan N. Brown, Christopher A. Barnes, Rena T. Kasick, Roger Michel, Thomas W. Gilbert,Donna Beer-Stolz, David G. Castner, Buddy D. Ratner, Stephen F. Badylak. Surface characterization of extracellular matrix scaffolds. Biomaterials 31 (2010), pp-428–437

2. Freezing of liver lobes(-80⁰C)

Cut in to slices of 5mm

Treat with enzymatic and detergent solutions

DI water wash for 30 min (3 times)

Treat with 0.02% trypsin/0.05% EDTA at 37⁰C for 1 h

Rinse in DI water

Treat with 3% Triton X-100 for 1hr

Place the slices in 4% sodium deoxycholic acid for 1 h

Rinse with DI water

Donald O. Freytes, Stephen F. Badylak, Thomas J. Webster, Leslie A. Geddes, Ann E. Rundell. Biaxial strength of multilaminated extracellular matrix scaffolds. Biomaterials 25 (2004), pp-2353–2361