Mathias Roth

9 Questions 18 Answers 0 Followers

Questions related from Mathias Roth

Is the measured area in an image with a bigger scale in Y than in X dependent of the orientation?

I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...

06 June 2018 1,264 6 View

How to deal with imprecise / vague information on time periods in statistics?

Part of the retrospective evaluation of a pathology "XY" is the question "Since when did you realize those symptoms?". As answers I get a variety of answers, like: - "since 6 days - since 2-3...

11 November 2017 2,802 5 View

How to analyze a qPCR with 2 or 3 housekeeping genes?

I want to analyze the results of a plate with a target gene and 2 or 3 housekeeping genes. How does it work? How to combine the housekeeping genes? How would the calculation (delta Ct; delta-delta...

12 December 2013 6,668 21 View

Can anyone help me with this molarity / dilution calculation?

I calculated it myself, with dilution calculators (Sigma) and let colleagues do it - everybody gets a different result. Can you help me? - Substance X - Molecular Weight 300 - Dilution for...

08 August 2013 1,053 2 View

What type of colony forming efficiency assay / CFU-F assay do you recommend for MSC?

There are several different methods described for CFU-F assays for MSC. E.g. in the book "Mesenchymal Stem Cell Assays and Applications" there are several partly very different methods described....

07 July 2013 9,903 5 View

Can you recommend a protocol and a retailer/producer for the ingredients of a "self-made" differentiation medium (adipo-/osteogenesis) for murine MSC?

I would like to mix the differentiation-medium (adipogenesis/osteogenesis) for murine MSC myself. I found several publications about this, basically it comes down to: Basal medium + FCS +...

07 July 2013 5,516 6 View

Can anyone recommend a protocol for the acellularization of a liver?

I would like to try to acellularize a rats liver. How to do it? What medium do you use for the perfusion? What max. pressure do you use?

07 July 2013 8,278 6 View

Is it possible to do a flow cytometry / facs analysis of cells DIRECTLY after they were solved out of glandular tissue?

I wonder if it is possible to dissociate glandular tissue by an agent like collagenase / accutase etc. and do an flow cytometry / cell sorting right afterwards with those cells. Or do you think...

07 July 2013 6,265 9 View

Can anyone recommend a good CD105 antibody for immunofluorescence in mice?

I am looking for a working CD105 AB for IF in mice. I tried one from Millipore but it did not work for me.

04 April 2013 9,734 0 View