Can anyone recommend a good aparatus for performing protoplast fusion?

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Livro DNA Sequencing Protocols de Colin A Graham ?

Gostaria de saber se alguém teria este livro em pdf, o DOI ao qual tive acesso não gerou resultados.

11 June 2024 4,042 0 View

What is the best fertilizer to use when growing potatoes?

Add

18 April 2024 8,327 3 View

Any suggestions for neuronal transfections?

I am currently culturing hippocampal neurons isolated from E18 rats and grown in Neurobasal medium (not the plus version) supplemented with B27 Plus. I transfect these neurons around DIV9 with...

06 November 2023 7,284 0 View

How does collaborative approach help clinical supervision of teachers?

contextualize in Caribbean setting, comparing research done in other parts of the world

18 October 2023 5,341 0 View

Hi, can someone please enlighten me on why we use IL-12 and IL-18 stimulation on human NK cells for checking IFN-gamma production?

I want to check the cytotoxicity by measuring IFN-gamma production of human primary NK cells after co-incubation with a fungal pathogen.

27 June 2023 9,092 2 View

Theory behind the Liebermann-Burchard assay for saponin determination?

Hi all I am interested to determine the saponin content from oily seeds and have found a few methodology from papers that uses Liebermann-Burchard assay (via UV-Vis spectrophotometer), however I...

15 May 2023 4,889 0 View

Extraction solvent of saponins from oily seeds?

Hi all I am currently embarking a project to determine saponin content in oily seeds, I have been looking at various literature methodology and according to available data in the literature,...

15 May 2023 1,261 1 View

Is sigma square considered model parameter in simple linear regression?

Some inclue only slope and intercept, but some include variance too as a model parameter. what is right?

10 March 2023 796 1 View

Hi, does anyone have experiences with the transfection of Primary Nk cells?

I want to do it but I'm a bit concerned about the viability at the end.

12 February 2023 7,114 2 View

SPSS statistical test advice?

Hello, I hope you are all well. I am finishing my MSc and am struggling quite a bit with organising/ analysing my data. I have conducted a study looking at the effect of different model sizes on...

15 August 2021 7,558 3 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View