Question cancelled Question cancelled ?

Question cancelled

19 January 2021 7,478 3 View

Multiple sequence alignment - polymorphism for restriction digest?

Hi, After comparing multiple (2+) DNA sequences, I want to identify ONLY the restriction sites that changed due to the detected polymorphism. I tried several things by hand, but it's WAY too...

23 September 2020 6,289 4 View

Would you be interested in the special issue "High Temperature Statistical Thermodynamics of Molecules in Gases and Plasmas", Entropy IF=2.494?

https://www.mdpi.com/journal/entropy/special_issues/Gases_and_Plasmas

26 August 2020 9,474 4 View

Which antibodies are recommended for CTLA-4, Tigit, Lag-3 and Tim-3?

I am new to immune oncology. What are the best antibodies against CTLA-4, Tigit, Lag-3 and Tim-3 for IHC and Flow cytometry? Are there any problems I need to be aware of?

19 August 2020 6,258 4 View

Can I run a chi-square test when my data include one 'zero' value? Can I run a post hoc analysis after chi-squre when p<0.05?

I have one row of raw data 13, 0, 24, 26, 17 and 14. It is a larval survival rate / six treatments. Can I run chi-squared with zero inluded? I use R, and it looks like it works, but I am not...

24 July 2020 7,720 5 View

Immobilizing an antibody with Protein A/G for SPR?

I am planning to perform an antibody-ligand interaction study with SPR/Biacore. The idea is to immobilize my antibody with Protein A/G. It would be great if you could share your comments, what to...

23 June 2020 7,705 3 View

How to identify crystals in the shrink cavity?

I have a problem. I watched the crystals on the surface of the shrinking cavity. How to recognize these elements? EDS analysis is not possible due to the depth of the shrink cavity. Machining can...

29 May 2020 4,235 0 View

How to choose the right protein subunit for symmetrical docking?

Recently, I have been dealing with symmetrical docking of protein complexes and I have a problem. Well, I have no problems with docking complexes with cyclic symmetry, it is double-walled symmetry...

09 May 2020 4,424 3 View

How do I compare regression coefficients in terms of effect size?

Hi everyone, I have two distinct samples, and in both samples I am examining the relation between age and income. I therefore conduct simple regression analyses (where y represents income and x...

18 February 2020 3,763 3 View

How to model a material in Abaqus with 2 types of behavior: high stiffness in compression and low stiffness in tension?

I am modeling a dynamic problem in Abaqus. The material should have two types of properties: different Young's moduli in tension and compression. Any simple way to achieve it? Thanks!

17 February 2020 8,563 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View