Marcin Schmidt

6 Questions 25 Answers 0 Followers

Questions related from Marcin Schmidt

Can anyone suggest AFLP selective primers for fingerprinting of specific genera?

I need to perform AFLP fingerprinting of: Lactobacillus, Lactococcus, Enterococcus, Leuconostoc, Carnobacterium, Streptococcus, Weisella and Pediococcus isolates. Can anyone suggest an AFLP...

13 July 2015 5,467 3 View

Can anyone recommend a detailed protocol for agglutination of yeast cells with bacterial cells?

I am looking for a detailed protocol to analyse the ability of probiotic bacteria, e.g. Lactobacillus sp. to agglutinate with yeast cells (Saccharomyces cerevisiae). Can you recommend one? Also,...

08 January 2015 6,162 0 View

Has anyone used Ultra-Deep Microbiome Prep? How does it work regarding isolating DNA from live bacteria?

I want to isolate DNA from live bacteria only to analyse bacterial community dynamics. Has anybody experience in 'differential isolation' from live cells only. I have found Ultra-Deep Microbiome...

07 October 2014 731 3 View

Is it possible to differentiate, with a simple qPCR assay, a mature B-cell from a non-B-cell?

I need to differentiate human mature B-cells from non-B-cells (including pre-B-cells), and I would prefer to use a simple qPCR genomic DNA based assay e.g. depending on the amplification of a...

05 April 2013 6,103 8 View

Using white plates for Applied Biosystems 7500 Real-Time PCR

Has anybody tried or use white plates (non-transparent) for Applied Biosystems 7500 Real-Time PCR machine. Are the plates provide better performance? What about background calibration?

29 March 2013 9,430 6 View

KEGG pathways custom coloring genes

I am looking for simple way to mark in two colors objects (genes, proteins) in KEGG pathway maps. Just to show that the same gene is upregulated by one and downregulated by other treatment. Not...

05 September 2012 5,267 7 View