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19 January 2021 7,478 3 View

Multiple sequence alignment - polymorphism for restriction digest?

Hi, After comparing multiple (2+) DNA sequences, I want to identify ONLY the restriction sites that changed due to the detected polymorphism. I tried several things by hand, but it's WAY too...

23 September 2020 6,289 4 View

Would you be interested in the special issue "High Temperature Statistical Thermodynamics of Molecules in Gases and Plasmas", Entropy IF=2.494?

https://www.mdpi.com/journal/entropy/special_issues/Gases_and_Plasmas

26 August 2020 9,474 4 View

Can I run a chi-square test when my data include one 'zero' value? Can I run a post hoc analysis after chi-squre when p<0.05?

I have one row of raw data 13, 0, 24, 26, 17 and 14. It is a larval survival rate / six treatments. Can I run chi-squared with zero inluded? I use R, and it looks like it works, but I am not...

24 July 2020 7,720 5 View

How to identify crystals in the shrink cavity?

I have a problem. I watched the crystals on the surface of the shrinking cavity. How to recognize these elements? EDS analysis is not possible due to the depth of the shrink cavity. Machining can...

29 May 2020 4,235 0 View

How to choose the right protein subunit for symmetrical docking?

Recently, I have been dealing with symmetrical docking of protein complexes and I have a problem. Well, I have no problems with docking complexes with cyclic symmetry, it is double-walled symmetry...

09 May 2020 4,424 3 View

How to model a material in Abaqus with 2 types of behavior: high stiffness in compression and low stiffness in tension?

I am modeling a dynamic problem in Abaqus. The material should have two types of properties: different Young's moduli in tension and compression. Any simple way to achieve it? Thanks!

17 February 2020 8,563 2 View

How many water molecules are incorporated into the structure of fructan?

I am looking for help to calculate how many water molecules are incorporated into the structure of fructan. We want to apply polymer density functional theory (DFT) for prediction and description...

01 April 2019 7,096 3 View

No title

I test preference and survival rate of one population (moth larvae). I have six varieties of plants (a-f) in six replicates (36 in total) arranged in Latin square block. I applied 5 larvae (1st...

04 March 2019 5,183 3 View

How to understand "no change in luminescence band" when I can see this change?

26 June 2017 5,786 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

Should I make 2 helical antennas? One for 2.4 GHz and another for 5.8 GHz?

Should I make 2 antennas, one for 2.4 GHz and one for 5.8 GHz? How should I approach the problem if I want to design the antennas which cover 400 and 900 MHz and 2.4 and 5.8 GHz? All together in...

26 January 2021 906 3 View

SPSS - PLUM: Shall I introduce binary variables as covariates?

I am doing an analysis of interaction effects, using PLUM in SPSS. My question is if the binary variables, that i have constructed to analyze the interaction effects, should be introduced as...

17 January 2021 8,217 4 View

Guidance in choosing a column for UHPLC quantification of organic acids in cranberry and blueberry fruit?

07 January 2021 6,214 3 View

How aromatics can dissolve asphaltene ?

As we all know asphaltene is defined as " The highest molecular weight component in the crude oil that is insoluble in light n-alkanes such as n-pentane or n-heptane and soluble in aromatics such...

05 January 2021 6,822 2 View

Do you advice me how I can dissolve samples with different polarity in a culture media such as demem or emem??

We want to dissolve our research samples in cell culture media such as demem or emem. We want to conduct bio-assay guided technique to isolate bio-active compounds. But we have a difficulty to...

30 December 2020 3,590 3 View

How to clean a packed glass column with C18?

I loaded a polar fraction on a glass column packed with C18 and washed it isocratically with 10% methanol for 6 days. The color of this fraction remained on top of the column, then I washed it...

28 December 2020 7,884 3 View

Which polar protic solvent are used in replacement of Ethanol or methanol?

In reaction condition, my starting materials are highly polar which are not soluble in ethanol and methanol.

03 December 2020 5,792 2 View

How do I desalt soy sauce so as to analyse for UPLC-Q-TOF-MS?

Hello everybody, there is a problem plagued me for a long time. My interest is to identify some polar amino acids and their derivates by UPLC-MS/MS. While, due to the high content of salt in soy...

18 November 2020 7,814 4 View