https://www.omicsonline.org/scientific-reports/2157-7463-SR-404.pdf

http://www.academicjournals.org/article/article1380539663_Ahmad%2520et%2520al.pdf

Article Production of Biodiesel Fuel from the Microalga Schizochytri...

http://onlinelibrary.wiley.com/doi/10.1002/ejlt.201100106/full

Article Comparison of direct transesterification of algal biomass un...

Article Importance of algae oil as a source of biodiesel

Hi jyoti, the best method for transesterification depend on the Acid value.If the Acid value is low a transesterification with sodium hydroxide as a catalyst is enough. If the Acid value is high you require two different reaction steps, a first reaction step using sulfuric acid as a catalyst in order to reduce the Acid value and a second reaction step with sodium hydroxide.

Thank you so much Hamit can sir for sharing the link.

Dear Mario Andres Noriega sir what if I donot know the acid value? I have used acid catalysed single step transesterification process but am unable to seperate the oil layer.

Hello Jyoti,

You can follow the attached protocol.

Dear Monjurul Haq thank you so much for sharing the protocol.

Dear Jyoti, it is possible that you are unable to separate the oil because the reaction did not achieve the maximum conversion, the acid reaction needs more time than the alkali reaction, so, a good idea is to increase your reaction time and temperature. Again, I encourage you, to evaluate the acid value, it is an easy technique an improve your results explanation. Good Luck.