I am trying to find an indirect method/protocol for metallothionein estimation (non- radioactive method) in tissues. Please kindly help.
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...
01 August 2024 8,217 1 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
In research papers on beam steering antennas, while the radiation pattern for the beam scanning plane (such as the horizontal or vertical plane) is typically presented, details regarding the...
03 July 2024 8,321 0 View
If I want to increase the photocatalytic activity of a thin film sample what should be it's ideal surface morphology and parameters? All scientific answers related this are highly appreciated.
19 June 2024 6,744 0 View
>Error # 7202. Command name: GET STATA >Input dictionary read error. >Execution of this command stops. Cross Product Matrices are not supported
19 June 2024 5,423 0 View
If I want to improve the photocatalytic activity of Ni doped ZnO thin film then which would be the better deposition process for thin film Spin Coating or Dip Coating?
18 June 2024 7,678 2 View
If I want to enhance the photocatalytic activity of Ni doped ZnO thin film. For this what are the parameters I need to tune and how? Related this all scientific answers/explanations are highly...
16 June 2024 4,533 1 View
Also, I will appreciate whole curing mechanism of Novolak with Hexamine
14 June 2024 9,908 0 View
From the definition of cell line, they are indefinitely propagated/subcultured. However, in practice beyond a certain passage number, they are said to loose characteristic gene profile, for e.g....
10 June 2024 6,890 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View