Can anyone please elaborate the method for candida whole proteome extraction using zymolyase and glass beads?

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What is wrong with this 2D protein gel?

Hello All, Here I have attached a 2D protein gel (silver stained) image. Can anyone help me with the expert comments on this gel? It would be a great help. Thank you in advance.

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Why does proteins at lower molecular weight loose staining after complete destaining?

I am running a 12% SDS-PAGE gel with whole cell lysates. The problem is with lower molecular weight proteins (lower 1/4th of the gel) which lost their visibility after destaining. What could be...

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Why are 2D spots coming in short pH range?

Hello Researchers, I am working with yeast whole proteome but after a lot try I only get spots in region between pH 4-6 using pH 3-10, 7cm strip, following BIO-RAD standard protocol. What could be...

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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How to increase a model accuracy ?

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How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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Brijesh Mishra

HI,

Apurva.

please find the attachment it may help you

Rabiul Islam

Dear Apurva Chatrath,

You can use to salting out method. In this method use ammonium sulphate and separation any type protein are easily.

Zeena Fouad

Dear Apurva Chatrath

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182553