Apurva Chatrath

4 Questions 6 Answers 0 Followers

Questions related from Apurva Chatrath

What is wrong with this 2D protein gel?

Hello All, Here I have attached a 2D protein gel (silver stained) image. Can anyone help me with the expert comments on this gel? It would be a great help. Thank you in advance.

07 July 2017 1,877 4 View

Can anyone please elaborate the method for candida whole proteome extraction using zymolyase and glass beads?

I have been using glass beads for protein extraction but protein quantity is low. If anyone can help with tried protocol of candida proteome extraction?

03 March 2017 6,340 3 View

Why does proteins at lower molecular weight loose staining after complete destaining?

I am running a 12% SDS-PAGE gel with whole cell lysates. The problem is with lower molecular weight proteins (lower 1/4th of the gel) which lost their visibility after destaining. What could be...

02 February 2017 4,445 10 View

Why are 2D spots coming in short pH range?

Hello Researchers, I am working with yeast whole proteome but after a lot try I only get spots in region between pH 4-6 using pH 3-10, 7cm strip, following BIO-RAD standard protocol. What could be...

06 June 2016 2,277 12 View