It looks like it's been warped by excessive heating to me.

See page 126-128 of the GE healthcare troubleshooting. You can get all info and solution over there.

This can be due to

1. Horizontal streaking- cause:Non-protein impurities in the sample can interfere with IEF, causing horizontal streaking.

solution: a.Modify sample preparation to limit these contaminants (see section 1.4).

Use 2-D Clean-Up Kit (section 1.4.1). b.The effect of ionic impurities can be reduced by modifying the IEF protocol. Limit the voltage to 100-150 V for 2 h, then resume a normal voltage step program. This allows the ions in the sample to move to the ends of the Immobiline DryStrip gel.

2. Impurities in rehydration (see page 55 of the attached)- cause: Impurities in rehydration solution components. solution: Use only high-quality reagents. Deionize urea solutions. 

The suggestions above seems quite reasonable, you should definitely  check the voltage and maybe run the gel slowly, also be sure the running buffer is the same as the gel. 

I suppose your gel image is upside down. Is the alkaline part of the pH gradient on the left? It seems that most of your problems occur during the IEF, e.g. as Aayudh already indicated these are probably still discturbing impurities in the samples or might be also in part not fully reduced proteins (too low DTT concentration in sample and rehydration buffer?). Thus, to give you some more hints we would need your detailed protocols and the right orientation of the 2D gel.