I am currently conducting a research into fungal enzymes involved in glycosyl hydrolysis. I am to select one of three organisms for enzymes production. The activity of the enzyme produced by the best of these three organisms is twice the second best and three times the least. However, when I used the same enzyme units (30FPU) for the three organism in a target hydrolysis, enzymes from the other two organisms (those with lower activities) had higher sugar yields (10% total sugar yield more than the best organism). Of course, I thought that the other two organisms might posses some other complementary enzymes which act in synergy to produce a higher sugar yield but I am concerned that they however require a higher volume of crude enzymes or some expensive enzyme concentration mechanisms that will rather make the hydrolytic process non cost effective. I urgently need a decisive guide about this.
Choosing the best enzyme is not an easy task as many other factors rather than enzyme activity may have effect on product formation.I think you need to have some information about the metabolism of these three yeasts.If their genome has been sequenced you can see which enzymes play important rule in final sugar yeild
Enzyme activity by set protocol and applying these enzymes for particular target reaction are totally different things. Factors influencing the target reaction might have significant effect on the enzyme from your three organisms. So I suggest choose your organism based on the actual performance in your target reaction and then further investigate the parameters influencing the enzyme activity such as temperature, pH, solvents, buffer etc.
hi good attempt. first you should consider specific enzyme activity for hydrolysis. consider the organisms which is producing more enzymes and do hydrolysis process , then check sugar yield. like wise see other organism for specific hydrolysis for substrate and calculate the sugar yield. compare the substrate specificity of your need, then choose your organisms. all the best
Your decision will be dependent on the parameters that you optimize or choose based on your experiments and study for the given organism and the enzyme performance. It may require more efforts to decide finally on the enzyme as suggested by our other researchers. All the Best.
@ Dr Ghosh, my concerns had been expressed in my question. I am actually not isolating enzyme but selecting the best organisms by a set of protocols for their enzyme activity and a reaction using their enzyme products for target hydrolysis.
Suggestions, especially by @ Guldhe and others, have actually been very helpful.
@ all, Thanks agin
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