I'm working with human osteosarcoma cell lines. I would like to know is there any morphological cues with which we can say the cell is undergoing differentiation? Apart from staining.
Dear Raj
These articles may be useful for you:
1) Effects of the myeloid cell nuclear differentiation antigen on the proliferation, apoptosis and migration of osteosarcoma cells.
Sun C, Liu C, Dong J, Li D, Li W.
Oncol Lett. 2014 Mar;7(3):815-819
2) Expression levels of insulin receptor substrate-1 modulate the osteoblastic differentiation of mesenchymal stem cells and osteosarcoma cells.
Contaldo C, Myers TJ, Zucchini C, Manara MC, Chiodoni C, Colombo MP, Nicoletti G, Lollini PL, Li T, Longobardi L, Scotlandi K, Spagnoli A.
Growth Factors. 2014 Feb;32(1):41-52
3) The tankyrase-specific inhibitor JW74 affects cell cycle progression and induces apoptosis and differentiation in osteosarcoma cell lines.
Wessel Stratford E, Daffinrud J, Munthe E, Castro R, Waaler J, Krauss S, Myklebost O.
Cancer Med. 2013 Dec 17. doi: 10.1002/cam4.170
4) Osteosarcoma cells promote the production of pro-tumor cytokines in mesenchymal stem cells by inhibiting their osteogenic differentiation through the TGF-β/Smad2/3 pathway.
Tu B, Peng ZX, Fan QM, Du L, Yan W, Tang TT.
Exp Cell Res. 2014 Jan 1;320(1):164-73
hi raj. in morphological ground, the nuclei of the neoplastic cell is a good reference of the differentiation; hipercromatic and pleomorphic nuclei and neoplastic osteoid is a signal of osteoblastic differentiation, if the tumor cell have a big nuclei, nucleoli, clear citoplasm or binucleation in a chondroid stroma, the differentiation is chondroid and finally a fusiform nuclei in a fibrous stroma is signal of fibroblastic differentiation.
greetings.
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