Can anyone help with retroviral mediated transfection control?

03 March 2014 0 6K Report

Viral mediated gene delivery is a common practice to transduce normal cells into cancerous cells, such as the transfection of hematological stem cells with MSCV-BCR-AB-GFP-IRES vector. My question is how do we control the rate of cell transfection (some cells may have single copy while other more than one) as increase in copy number may result in resistance against therapy?

Badges
Science topic

More Abdul Mannan's questions See All

Does anyone has experience of doing MCF7 tamoxifen cytotoxicity assay?

07 August 2017 126 5 View

Is it necessary to implant slow release estradiol (E2) pellet SC for BT474 xenografts?

We are planning to do BT474 xenografts in Nude mice. Though most of the article mentioned of using E2 for BT474 xenografts, I came across some other article in which the use of E2 was not...

04 May 2017 2,720 1 View

How important it is to grow MCF7 in phenol red free medium while doing Tamoxifen treatment experiment?

03 April 2017 4,524 4 View

If ZR-751 is a luminal A or B cell line?

We are working on breast cancer with the main focus on luminal B tumours. We have been using BT474, ZR751 and ZR7530 cell lines as a model for luminal B breast tumour, but recently I came across...

31 December 2016 8,634 2 View

Can anyone help me with the Soft Agar Assay?

We are trying to do soft agar assay in BT474 (luminal B) breast cancer cells. I tried couple of times, but could not get even a single colony. Last time I even tried using MCF10A-Ras cells but...

02 March 2016 3,844 10 View

Can anyone help me with a Transwell matrigel invasion assay and wound healing assay on BT474 Breast cancer cells?

11 December 2015 6,869 7 View

Can anyone help in figuring out why we are having broader and sometime multiple peaks with cell cycle analysis?

We are doing cell cycle analysis in ZR-751 cell lines. We seed cells @ 5x105 cells per well in six well plate on day 1. On day-3 we are treating cells with DNA damaging agent for 1 hour followed...

06 July 2015 6,680 5 View

Are you familiar with optimizing an Luciferase Assay?

03 April 2015 6,520 3 View

Any advice on MCF-7 Soft Agar Assay colony formation and crystal staining?

03 April 2015 8,293 1 View

Why do I get this problem in my soft agar assay cell feeding?

02 March 2015 3,240 7 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View