Hi,
I am trying to prepare PLGA based nanoparticles for hydrophilic drugs. I tried double emulsion solvent evaporation technique which resulted in only 0.5 % loading capacity and 20% entrapment. The size of the particles was approx 150nm. I want to enhance the loading capacity of the hydrophilic drug in the PLGA nanoparticles with the size range approximately similar.
Please suggest me better methods to ehance the encapsulation of the hydrophilic drug in these PLGA nanoparticles.
Thanks.
See this Demo in JOVE...hope this would help you
http://www.jove.com/video/51015/plga-nanoparticles-formed-single-or-double-emulsion-with-vitamin-e
You should be able to load hydrophilic drugs into PLGA nanoparticles by using the double emulsion solvent evaporation method. My experience is that you can optimize the method parameters to increase the loading, however, the maximum loading I have got has been in the range of 2-3 % and not more (this loading level was achieved for proteins). Please see the following publication which studies the effect of some parameters on the loading of hydrophilic peptides into PLGA nanoparticles.
Article Optimization of encapsulation of a synthetic long peptide in...
Thanks a lot... I am trying certain modifications.. will come again.. once I get some more results...
Hi! Did you get a higher loading? I´m facing the same challenge now. Have you tried to saturate the PVA phase for instance?
No, I couldn't get a good loading capacity for a hydrophilic drug using PLGA nanoparticles. A lot of drug gets wasted. People suggested that you can still do experiments with that little loading capacity... hence, I guess it's really not economical to use PLGA nanoparticles for hydrophilic drug loading.
I am unable to find the above article anywhere.. is it possible for you to send it to me? Best.