How to set up TETRA-ARMS PCR at higher temperatures?

Hello everyone .i'm trying to do TETRA ARMS PCR. My primers have TM :64 and one of the primers has TM :66(Reverse outer). How can I setup at higher temperatures to have mutant band? Because at 65...

03 August 2020 4,742 2 View

Can graphene layers be coated by CVD on hydroxyapatite powders?

Is the hydroxyapatite decomposed during the CVD process?

29 February 2020 5,443 3 View

What is characteristic peak(s) in FTIR spectrum of crude plant extract?

Hello, I synthesized chitosan nanoparticles loaded with a plant extract. For confirming presence of the extract in the nanoparticles I did a FTIR analysis. Now my question is: What is...

24 January 2020 4,402 2 View

How to ensure that a chemical synthesized peptide has correct sequence?

Dear all, I have ordered a peptide (with 32 aa) for chemical synthesis, but I dont know after receive a peptide how to ensure from its sequence. Which method(s) are available for my purpose?...

09 December 2019 3,784 3 View

How do I choose the major compound of Essential oil?

hi everybody, how do I choose the major compound of Essential oil ? is there any special index or scale for determining the major compound ? what method shall I use ? thanks.

06 December 2019 4,133 2 View

How we can delete some molecules from box in vmd?

I want to delete some solvent molecules in box after md production, but I don't the lables of them and how delete them and how I delete a specific molecule??

11 November 2019 6,415 1 View

How to measure Moderator variable with SPSS?

Dear researchers, I want to learn how to measure moderator variable with SPSS. If you have any resources or videos i will appreciate to share it with me.

23 June 2019 9,504 3 View

Can any one suggest best and easy method to measure Enzyme activity of glutaminase in breast cancer cell lines?

I search a lot to find the protocol assay for measurment of enzyme activity of glutaminase but i can not find the best method.please help me.

11 April 2019 2,976 2 View

Which copper precursor if preferred for cu-nanoparticles synthesis in a green synthesis method?

Hi every one, I would like to synthesis of Cu Nanoparticles using plant extract, but in previous works different precursors have been used ( chloride and sulfate salts) , based on your experience...

17 October 2018 5,926 3 View

Can anyone tell me how can I extract some number in text?

Hi, I have a text that include some number in latest line and I would like to extract this numbers to a separate file. Can anyone help me in this regard. A sample file of mu results is attached....

29 May 2018 3,620 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View