I cultured human CD34+ cells in 96 # 7days for randomly differentiated. Some cells(I am not sure…) are “bold". What are those big cells? I marked them in the pdf file.
Can you take a closer picture please (higher magnification)? It's hard to look at the morphology.
1.I want to isolate brown adipose from mice. I am not sure whether the tissue is right?(show in the attachment.) 2. I want to digest brown adipose to collected single cell sample. After 1 hour...
03 April 2018 2,783 0 View
I want to find the list of mice receptor ligand binding.
02 March 2018 6,109 3 View
1. western and RT PCR show the protein is high expression. 2.FCM show opposite result. 3.The antibody is different. ^^^^^The protein have been modified after translate?
11 December 2016 2,310 3 View
I want to do Ex Vivo Functional Analysis of human Dendritic Cells. What i found was here: 1,Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay (Principal...
09 October 2016 342 1 View
I got some NSG-like mice from company(in China). Those mice can survival in lab gangway(not clear) for almost 2 weeks. Does it normal? I want to check their blood through FCM. And I want to use...
08 September 2016 7,228 3 View
I want to check several genes in one single cell, What kit can i use?
08 September 2016 4,174 0 View
Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Hyde Chen on Sep 21, 2016
08 September 2016 9,749 3 View
I use the 4034 from stemcell. Seed cell are cd34+ from hES.
06 July 2016 4,462 4 View
I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...
06 July 2016 8,578 4 View
HSC are Cryopreserved. After thawing, HSC are used directly or culture on SFEM for a while before bone marrow transplantation(human HSC to mice)? Does Cryopreserving affect the activity of HSC.
06 July 2016 1,228 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View