Bovine serum albumin can detach A549 cells?

Hello all, I accidentally found that adding bovine serum albumin (BSA) in serum-free RPMI 1640 medium (1% w/v) caused A549 cell detachment. Have you known about that effect of BSA? I tried to find...

05 June 2016 9,659 2 View

What is the function of fetal bovine serum on degranulation of eosinophils, mast cells and neutrophils?

11 December 2015 9,577 3 View

Any advice on a MTT assay false positive result?

Hello all, I am using MTT assay to test the toxicity effect of a substance on A549 cells (adherent cell). I seed 5x10^4 cells/well of a 96 well-plate, incubate overnight for cell attachment. Then...

05 June 2015 6,982 10 View

How did you induce NET formation by IL-8 from neutrophils isolated from healthy donors?

Hello all, I am trying to induce NET induction by IL-8 (100 ng/ml) using healthy neutrophils and patients' neutrophils. I quantify the NET induction by Picogreen assay. In case of healthy...

04 May 2015 8,384 4 View

Does pressing hard when mounting cause cell morphology changes?

08 September 2014 9,158 2 View

Why do I get no plasmid after Maxiprep with MISSION shRNA plasmid DNA?

08 September 2014 3,793 11 View

Knockdown gene in LAD2 cells?

Hello all, I would like to knockdown some gene in LAD2 cells to produce a stable expressing cell line. Could you suggest me an appropriate method to do? Because LAD2 cells are stem cells and they...

05 June 2014 981 0 View

What is the appropriate voltage for transferring LC3 in western blot?

I am using 80V/90min to transferring LC3 from 12% SDS-PAGE to PVDF membrane. But recently, I have not gotten any band of LC3. As I've read somewhere that LC3 could swim through the membrane if we...

04 May 2014 159 13 View

I got 2 bands for beta-actin in Western Blot when I used human neutrophils, but not for other cell lines. How can I solve this problem?

When I performed WB with cell lysate from human neutrophils, I got 2 bands of beta-actin. For other cell lines (HMC-1, LAD2 cells) I just only got 1 band for b-actin. I used the same antibody and...

03 April 2014 3,006 0 View

Does anyone have experience performing Western Blot with a low molecular weight protein?

I used to performed Western Blot to detect a protein with #14-16 kDa molecular weight (LC3) a few months ago. It worked very well. I have stopped doing that experiment for one month. I am now...

02 March 2014 6,743 10 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View